期刊
CHEMICAL ENGINEERING JOURNAL
卷 412, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.128660
关键词
GVL-HCl pretreatment; Lignin auto-fluorescence; CBM-GFP binding; Confocal laser scanning microscopy (CLSM); Real-time imaging; Enzyme digestibility
资金
- U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-SC0019072]
- Great Lakes Bioenergy Research Cente [DE-SC0018409]
- U.S. Department of Energy (DOE) [DE-SC0019072] Funding Source: U.S. Department of Energy (DOE)
Thermochemical pretreatment is crucial for processing lignocellulosic biomass to produce biofuels and biomaterials. γ-Valerolactone (GVL) has been used as a green solvent to dissolve lignin effectively. This study compared the performance of HCl and H2SO4 as catalysts in GVL pretreatment, finding that GVL-HCl at 100°C showed similar effectiveness to GVL-H2SO4 at 120°C in terms of xylan and lignin removal. Real-time imaging revealed that GVL-HCl pretreatment at lower temperature could rapidly remove lignin from cell walls and optimize the efficiency of GVL-based pretreatment.
Thermochemical pretreatment is one of the key steps to process lignocellulosic biomass for production of biofuels and biomaterials..-valerolactone (GVL) has been used as a green and renewable solvent to efficiently dissolve lignin under acidic conditions and enhance subsequent enzyme digestibility. Further improvement is still required to lower the pretreatment temperature in order to reduce sugar degradation and irreversible lignin condensation, as well as the capital cost. In this study, we compared the use of HCl and H2SO4 as catalysts during GVL pretreatment, and found the performance of GVL-HCl at 100 degrees C was comparable to that of GVL-H2SO4 at 120 degrees C in terms of xylan and lignin removal and enzyme digestibility. We further monitored the lignin removal and cellulose accessibility in the cell walls at different pretreatment time points by imaging the changes in lignin auto-fluorescence and in CtCBM3-GFP binding with confocal laser scanning microscopy (CLSM), respectively. We found GVL-HCl pretreatment at a relative low temperature (100 degrees C) could rapidly remove lignin in the compound middle lamella and cell corner areas, which concurred with the increase of CtCBM3-GFP binding in these areas. Real-time imaging of cell wall degradation by cellulases further revealed that the secondary cell walls could be digested from both cell lumen and CML sides, and eventually fully deconstructed within 24 h. Our results provide new insight into the effects of chloride anions on plant cell wall structure during GVL pretreatment, and offer potential routes to further optimize and enhance the efficiency of GVL-based pretreatment.
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