期刊
CELLULAR AND MOLECULAR NEUROBIOLOGY
卷 42, 期 5, 页码 1373-1384出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10571-020-01022-x
关键词
Insulin-like growth factor-1; Spinal cord transection; Zebrafish; Motoneuron; Neuroinflammation
资金
- National Natural Science Foundation of China [81771384, 81801276]
- Postgraduate Research and Practice Innovation Program of Jiangsu Province [KYCX19_1893]
- Public Health Research Center at Jiangnan University [JUPH201801]
- National First-Class Discipline Program of Food Science and Technology [JUFSTR20180101]
- Chinese Postdoctoral Science Foundation [2018M630512]
- Wuxi Municipal Health Commission [1286010241190480]
The study revealed that IGF-1 enhances motoneuron survival and inhibits neuroinflammation in zebrafish spinal transection model, suggesting its potential involvement in motor function recovery.
Insulin-like growth factor-1 (IGF-1) is a neurotrophic factor produced locally in the central nervous system which can promote axonal regeneration, protect motoneurons, and inhibit neuroinflammation. In this study, we used the zebrafish spinal transection model to investigate whether IGF-1 plays an important role in the recovery of motor function. Unlike mammals, zebrafish can regenerate axons and restore mobility in remarkably short period after spinal cord transection. Quantitative real-time PCR and immunofluorescence showed decreased IGF-1 expression in the lesion site. Double immunostaining for IGF-1 and Islet-1 (motoneuron marker)/GFAP (astrocyte marker)/Iba-1 (microglia marker) showed that IGF-1 was mainly expressed in motoneurons and was surrounded by astrocyte and microglia. Following administration of IGF-1 morpholino at the lesion site of spinal-transected zebrafish, swimming test showed retarded recovery of mobility, the number of motoneurons was reduced, and increased immunofluorescence density of microglia was caused. Our data suggested that IGF-1 enhances motoneuron survival and inhibits neuroinflammation after spinal cord transection in zebrafish, which suggested that IGF-1 might be involved in the motor recovery.
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