4.7 Article

Ultrastructural modifications induced by SARS-CoV-2 in Vero cells: a kinetic analysis of viral factory formation, viral particle morphogenesis and virion release

期刊

CELLULAR AND MOLECULAR LIFE SCIENCES
卷 78, 期 7, 页码 3565-3576

出版社

SPRINGER BASEL AG
DOI: 10.1007/s00018-020-03745-y

关键词

Covid-19; SARS-CoV-2; Virus; cell interactions; Electron microscopy

资金

  1. INSERM
  2. University of Tours
  3. REACTIng consortium (INSERM)
  4. CNRS
  5. Institut Merieux

向作者/读者索取更多资源

Studies on SARS-CoV-2 have shown that early infection events involve clusters of single-membrane vesicles and annulate lamellae, with host cell-derived organelles transforming into virus factories later on.
Many studies on SARS-CoV-2 have been performed over short-time scale, but few have focused on the ultrastructural characteristics of infected cells. We used TEM to perform kinetic analysis of the ultrastructure of SARS-CoV-2-infected cells. Early infection events were characterized by the presence of clusters of single-membrane vesicles and stacks of membrane containing nuclear pores called annulate lamellae (AL). A large network of host cell-derived organelles transformed into virus factories was subsequently observed in the cells. As previously described for other RNA viruses, these replication factories consisted of double-membrane vesicles (DMVs) located close to the nucleus. Viruses released at the cell surface by exocytosis harbored the typical crown of spike proteins, but viral particles without spikes were also observed in intracellular compartments, possibly reflecting incorrect assembly or a cell degradation process.

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