期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 78, 期 6, 页码 3005-3020出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-020-03703-8
关键词
PDAC; Collagen; Extracellular matrix; Organoid; Exosome; miR-21; miR-195
资金
- Semmelweis University
- National Competitiveness and Excellence program [NVKP_16-0007]
- National Research, Development and Innovation Office, Hungary [OTKANN-118018]
- Higher Education Institutional Excellence Programme (Ministry for Innovation and Technology, Hungary)
- Semmelweis University Scientific and Innovation Fund [VEKOP-2.3.3-15-2016-00007, VEKOP-2.3.3-15-2017-00016]
Extracellular vesicles (EV) are considered a potential diagnostic tool for pancreatic ductal adenocarcinoma (PDAC), with PDAC patient-derived organoids serving as an ideal in vitro model for studying human cancers. The miRNA composition of EVs varies among patients, but there are common miRNA sets in matched organoids and blood plasma samples. Levels of miR-21 and miR-195 in PDAC blood EVs are elevated compared to healthy controls, suggesting a potential biomarker for the disease.
Extracellular vesicles (EV) are considered as a promising diagnostic tool for pancreatic ductal adenocarcinoma (PDAC), a disease with a poor 5-year survival that has not improved in the past years. PDAC patient-derived 3D organoids maintain the intratumoral cellular heterogeneity, characteristic for the tumor in vivo.Thus, they represent an ideal in vitro model system to study human cancers. Here we show that the miRNA cargo of EVs from PDAC organoids largely differs among patients. However, we detected a common set of EV miRNAs that were present in matched organoids and blood plasma samples of individual patients. Importantly, the levels of EV miR-21 and miR-195 were higher in PDAC blood EV preparations than in healthy controls, albeit we found no difference compared to chronic pancreatitis (CP) samples. In addition, here we report that the accumulation of collagen I, a characteristic change in the extracellular matrix (ECM) in both CP and PDAC, largely increases EV release from pancreatic ductal organoids. This provides a possible explanation why both CP and PDAC patient-derived plasma samples have an elevated amount of CD63 + EVs. Collectively, we show that PDAC patient-derived organoids represent a highly relevant model to analyze the cargo of tumor cell-derived EVs. Furthermore, we provide evidence that not only driver mutations, but also changes in the ECM may critically modify EV release from pancreatic ductal cells.
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