期刊
CELLS TISSUES ORGANS
卷 211, 期 3, 页码 304-312出版社
KARGER
DOI: 10.1159/000511764
关键词
Cultivated meat; Protein and lipid tunability; Cell sheet; Layer-by-layer biofabrication; Meat texture
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canadian Institute for Health Research (CIHR)
- Canada Research Chairs Program
The study demonstrates a new method for biofabricating muscle tissue, creating meat-like constructs without the need for exogenous materials. These constructs can be used as cultivated meat for consumption, as well as skeletal muscle models.
Global meat consumption has been growing on a per capita basis over the past 20 years resulting in ever-increasing devotion of resources in the form of arable land and potable water to animal husbandry which is unsustainable and inefficient. One approach to meet this insatiable demand is to use biofabrication methods used in tissue engineering in order to make skeletal muscle tissue-like constructs known as cultivated meat to be used as a food source. Here, we demonstrate the use of a scaffold-free biofabrication method that forms cell sheets composed of murine adipocytes and skeletal muscle cells and assembles these sheets in parallel to create a 3D meat-like construct without the use of any exogenous materials. This layer-by-layer self-assembly and stacking process is fast (4 days of culture to form sheets and few hours for assembly) and scalable (stable sheets with diameters >3 cm are formed). Tissues formed with only muscle cells were equivalent to lean meat with comparable protein and fat contents (lean beef had 1.5 and 0.9 times protein and fat, respectively, as our constructs) and incorporating adipocyte cells in different ratios to myoblasts and/or treatment with different media cocktails resulted in a 5% (low fat meat) to 35% (high fat meat) increase in the fat content. Not only such constructs can be used as cultivated meat, they can also be used as skeletal muscle models. (c) 2021 S. Karger AG, Basel
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