期刊
CELL CYCLE
卷 20, 期 2, 页码 179-193出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15384101.2020.1867782
关键词
GAS6-AS2; miR-144-3p; MAPK6; NSCLC; proliferation
类别
In this study, the new long non-coding RNA GAS6-AS2 was identified in non-small cell lung cancer (NSCLC) and its role in regulating proliferation of NSCLC cells was investigated. Results showed that GAS6-AS2 could function as a competitive endogenous RNA (ceRNA) by sponging miR-144-3p, further regulating the expression of MAPK6. Knockdown of GAS6-AS2 greatly suppressed tumor growth of NSCLC in vivo, suggesting GAS6-AS2 as an effective therapeutic target in NSCLC.
The function of a new long non-coding RNA GAS6-AS2 in non-small cell lung cancer (NSCLC) is not fully understood. In this study, GAS6-AS2 was identified, and its roles as well as mechanisms in regulating proliferation of NSCLCs cells were investigated. qRT-PCR was used to analyze GAS6-AS2, miR-144-3p, and MAPK6 expression. Protein expression was detected by Western blotting. Cell Counting Kit-8 (CCK8) assay was used to examine the cell proliferation ability. The interaction between GAS6-AS2 and miR-144-3p was confirmed by dual-luciferase reporter assay and RNA pull down assay. A xenograft model was constructed to monitor the mice NSCLC tumor growth in vivo. GAS6-AS2 was up-regulated, while miR-144-3p was suppressed in NSCLC cells compared with normal lung cells. GAS6-AS2 suppression could inhibit the progression of NSCLC cells, and miR-144-3p could attenuate the effect. GAS6-AS2 could function as a competitive endogenous RNA (ceRNA) via direct sponging miR-144-3p-3p, which further regulating the expression of MAPK6. The knockdown of GAS6-AS2 could greatly suppress the tumor growth of NSCLC in vivo. GAS6-AS2 up-regulated MAPK6 by sponging miR-144-3p in NSCLC tissues and cells. Thus, GAS6-AS2 is an effective therapeutic target in NSCLC.
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