期刊
CELL AND TISSUE BANKING
卷 22, 期 1, 页码 77-91出版社
SPRINGER
DOI: 10.1007/s10561-020-09867-8
关键词
Human adipose-derived stem cells; Exosomes; Osteogenic differentiation; Bone tissue engineering
资金
- National Natural Science Foundation of China [51872332, 31200740]
- National Natural Science Foundation of Liaoning province [20170541040]
Exosomes derived from human adipose-derived stem cells during osteogenic induction for 1-14 days efficiently promote osteogenic differentiation, proliferation, and migration of stem cells. Specific conditions of exosome treatment significantly enhance the expression of osteoblast-related proteins and are effectively internalized by cells.
Exosomes exhibit great therapeutic potential in bone tissue engineering. The study aimed to investigate whether the exosomes derived from human adipose-derived stem cells (hADSCs-Exos) during different time-span of osteogenic differentiation could promote osteogenesis. The appropriate concentrations of hADSCs-Exos to enhance the proliferation, migration and osteogenesis of hADSCs-Exos were also examined. PKH67 labelled hADSCs-Exos was used to detect the internalization ability of hADSCs. The osteogenic differentiation abilities of hADSCs after treatment with hADSCs-Exos was evaluated by Alizarin red staining (ARS). The proliferation and migration of hADSCs was examined by cell counting kit-8 and wound healing assay, respectively. The expression of exosomal surface markers and osteoblast-related protein of hADSCs was assessed by Western blot. PKH67-labelled exosomes were internalized by hADSCs after 4 h incubation. ARS showed that the amount of mineralized nodules in Exo(1-14d) group was significantly higher than that in Exo(15-28d) group. hADSCs-Exos could promote the proliferation and migration capacity of hADSCs. Western blot analysis showed that after hADSCs-Exos treatment, ALP and RUNX2 were significantly enhanced. Specially, the Exo(1-14d) group of 15 mu g/mL significantly upregulated the expression of RUNX2 than the other exosomes treated groups. Our findings suggest that exosomes secreted by hADSCs during osteogenic induction for 1-14 days could be efficiently internalized by hADSCs and could induce osteogenic differentiation of hADSCs. Moreover, administration of Exo(1-14d) at 15 mu g/mL promoted the proliferation and migration of hADSCs. In conclusion, our research confirmed that comprised of hADSCs-Exos and hADSCs may provide a new therapeutic paradigm for bone tissue engineering.
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