期刊
CELL
卷 183, 期 6, 页码 1665-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2020.10.026
关键词
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资金
- Connecticut Regenerative Medicine Research Fund
- Li Ka Shing Foundation
- Packard Fellowship for Science and Engineering
- Stand-Up-to-Cancer (SU2C) Convergence 2.0 Award
- Yale Stem Cell Center Chen Innovation Award
- NIH [U54CA209992, R01CA245313, UG3CA257393]
- Society for Immunotherapy of Cancer (SITC) Postdoctoral Fellowship
- Betty Ruth Hollander Scholarship
We present deterministic barcoding in tissue for spatial omics sequencing (DBiT-seq) for co-mapping of mRNAs and proteins in a formaldehyde-fixed tissue slide via next-generation sequencing (NGS). Parallel microfluidic channels were used to deliver DNA barcodes to the surface of a tissue slide, and crossflow of two sets of barcodes, A1-50 and B1-50, followed by ligation in situ, yielded a 2D mosaic of tissue pixels, each containing a unique full barcode AB. Application to mouse embryos revealed major tissue types in early organogenesis as well as fine features like microvasculature in a brain and pigmented epithelium in an eye field. Gene expression profiles in 10-mu m pixels conformed into the clusters of single-cell transcriptomes, allowing for rapid identification of cell types and spatial distributions. DBiT-seq can be adopted by researchers with no experience in microfluidics and may find applications in a range of fields including developmental biology, cancer biology, neuroscience, and clinical pathology.
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