期刊
CARBOHYDRATE POLYMERS
卷 251, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.117093
关键词
Pectin; Toll-like receptor 2; Degree of methyl-esterification; Degree of blockiness
资金
- Agrifirm Innovation Center B.V.
- Cooperatie Avebe U.A.
- DSM Food Specialties B.V.
- VanDrie Holding N.V.
- Sensus B.V.
- The Netherlands Organisation for Scientific Research (NWO)
Research has shown that pectins exhibit anti-inflammatory effects by inhibiting Toll-like receptor (TLR), and the inhibitory effect depends on the degree of methyl-esterification (DM) and distribution of methyl-esters over the galactumnic-acid (GalA) backbone (Degree of Blockiness - DB). Pectins with high DB and low DM can strongly inhibit TLR2-1, and can also inhibit IL-6 secretion in human macrophages. The anti-inflammatory effects of pectins are attributed to the number and blockwise distribution of non-esterified GalA.
Pectins have anti-inflammatory effects via Toll-like receptor (TLR) inhibition in a degree of methyl-esterification-(DM)-dependent manner. However, pectins also vary in distribution of methyl-esters over the galactumnic-acid (GalA) backbone (Degree of Blockiness - DB) and impact of this on anti-inflammatory capacity is unknown. Pectins mainly inhibit TLR2-1 but magnitude depends on both DM and DB. Low DM pectins (DM18/19) with both low (DB86) and high DB (DB94) strongly inhibit TLR2-1. However, pectins with intermediate DM (DM43/ DM49) and high DB (DB60), but not with low DB (DB33), inhibit TLR2-1 as strongly as low DM. High DM pectins (DM84/88) with DB71 and DB91 do not inhibit TLR2-1 strongly. Pectin-binding to TLR2 was confirmed by capture-ELISA. In human macrophages, low DM and intermediate DM pectins with high DB inhibited TLR2-1 induced IL-6 secretion. Both high number and blockwise distribution of non-esterified GalA in pectins are responsible for the anti-inflammatory effects via inhibition of TLR2-1.
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