Patterned dextran ester films as a tailorable cell culture platform

The elucidation of cell-surface interactions and the development of model platforms to help uncover their underlying mechanisms remains vital to the design of effective biomaterials. To this end, dextran palmitates with varying degrees of substitution were synthesised with a multipurpose functionality: an ability to modulate surface energy through surface chemistry, and an ideal thermal behaviour for patterning. Herein, dextran palmitate films are produced by spin coating, and patterned by thermal nanoimprint lithography with nano-tomicroscale topographies. These films of moderately hydrophobic polysaccharide esters with low nanoscale roughness performed as well as fibronectin coatings in the culture of bovine aortic endothelial cells. Upon patterning, they display distinct regions of roughness, restricting cell adhesion to the smoothest surfaces, while guiding multicellular arrangements in the patterned topographies. The development of biomaterial interfaces through topochemical fabrication such as this could prove useful in understanding protein and cell-surface interactions.

Automated summary

The study synthesised dextran palmitates with a multipurpose functionality to modulate surface energy through surface chemistry and to be patterned, and produced films by spin coating and patterned by thermal nanoimprint lithography. These moderately hydrophobic films performed well in cell culture and guided multicellular arrangements on specific topographies after patterning.