Polysaccharides from Pleurotus eryngii: Selective extraction methodologies and their modulatory effects on THP-1 macrophages

Polysaccharides from P. eryngii mushroom were selectively extracted using low-cost technologies (water at different conditions of temperature and pressure). Mannogalactan was the main polysaccharide in cold-water extracted fraction (CWEF), while a linear (1 -> 6)-beta-D-glucan was the main polymer in hot-water extracted fraction (HWEF). Autoclave-extracted fraction (AEF) contained a mixture of at least four different alpha and beta-glucans. The report of linear (1 -> 6)-beta-glucan and linear (1 -> 3)-beta-glucan is a new finding for P. eryngii fruiting bodies. The immunostimulatory properties of the fractions on THP-1 macrophages were studied. All fractions at 50, 250 and 500 mu g/mL were not cytotoxic and produced different stimulus on NO, IL-1 beta and IL-10 secretion by the cells. Thus, our results showed that it is possible to concentrate different P. eryngii polysaccharides in selected fractions using a simple and low-cost procedure. Since biological effects depends on the polysaccharide structure, this technique allows the obtainment of fractions with distinct immunomodulatory activities.

Automated summary

Polysaccharides from P. eryngii mushroom were extracted using low-cost technologies, resulting in different fractions with immunostimulatory properties on THP-1 macrophages. The main polysaccharides included Mannogalactan and various β-glucans, which were not cytotoxic and induced different cellular responses. This simple technique allows the obtainment of fractions with distinct immunomodulatory activities, showing the potential for future biological effects research.