Facile synthesis and antibacterial activity of geraniol conjugated chitosan oligosaccharide derivatives

A new chitosan oligosaccharide derivative (COS-N-Ger), based on geraniol (Ger) modificated onto the NH2 position of chitosan oligosaccharide (COS) via a facile method, was prepared and employed to evaluate in vitro antibacterial activity. The structures of COS-N-Ger derivatives were confirmed by FT-IR, H-1, C-13 NMR, and elemental analysis. The characterization results showed successful synthesis of derivatives and the degrees of substitution (DS) of COS-N-Ger1-3 were from 0.260 to 0.283 with the yields up to 78 %. The in vitro antibacterial activity evaluation results presented a significant inhibition effect of COS-N-Ger1-3 derivatives on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) as compared to COS. Moreover, their antibacterial activities were dose-dependent and more sensitive to S. aureus than E. coli. The results provide reliable theoretical supports for exploring the application of COS derivatives in the food industry as new potential antibacterial agents.

Automated summary

A new chitosan oligosaccharide derivative modified with geraniol was synthesized and found to exhibit significant inhibitory effects on Escherichia coli and Staphylococcus aureus, with greater sensitivity towards S. aureus. The results support the potential application of COS derivatives as novel antibacterial agents in the food industry.