期刊
CANCER CELL INTERNATIONAL
卷 21, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12935-020-01743-5
关键词
Hydrogen; Gastric cancer; LncRNA MALAT1; miR-124-3p; EZH2; Proliferation; Migration
类别
Hydrogen gas treatment significantly inhibits proliferation and migration of gastric cancer cells, while affecting the expression of related genes, showing potential therapeutic effects. In addition, the lncRNA MALAT1/miR-124-3p/EZH2 axis may serve as an important target for intervention.
BackgroundGastric cancer is one of the most prevalent and deadly malignancies without efficient treatment option. This study aimed to investigate the effect of hydrogen gas on the behavior of gastric cancer cells.MethodsGastric cancer cell lines MGC-803 and BGC-823 were treated with or without H-2 /O-2 gas mixture (66.7%:33.3% v/v). Proliferation and migration were assessed by MTT and scratch wound healing assays respectively. The expression of lncRNA MALAT1, miR-124-3p, and EZH2 was analyzed by real-time quantitative PCR and/or western blot. Tumor growth was estimated using xenograft mouse model.ResultsH(2) gas significantly inhibited gastric tumor growth in vivo and the proliferation, migration, and lncRNA MALAT1 and EZH2 expression of gastric cancer cells while upregulated miR-124-3p expression. LncRNA MALAT1 overexpression abolished all the aforementioned effects of H-2. LncRNA MALAT1 and miR-124-3p reciprocally inhibited the expression of each other. MiR-124-3p mimics abrogated lncRNA MALAT1 promoted EZH2 expression and gastric cancer cell proliferation and migration.ConclusionsThese data demonstrated that H-2 might be developed as a therapeutics of gastric cancer and lncRNA MALAT1/miR-124-3p/EZH2 axis could be a target for intervention.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据