期刊
BRITISH JOURNAL OF NUTRITION
卷 126, 期 6, 页码 825-836出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S000711452000481X
关键词
Pomegranate peel extract; Microencapsulation; Obesity; Mitochondrial function; Mitochondrial cristae
资金
- Departamento de Nutricion, Facultad de Medicina, Universidad de Chile
- FIA [PYT-2018-0309]
- FONDECYT [11169541]
- Unidad de Microscopia Avanzada, Universidad Catolica, Chile
- Agencia Nacional de Investigacion y Desarrollo (ANID)
- Agencia Nacional de Investigacion y Desarrollo (FONDECYT) [1171550]
- Agencia Nacional de Investigacion y Desarrollo (CONICYT-PFCHA/Doctorado Nacional) [2017-21170196]
The study demonstrates that microencapsulated pomegranate peel extract can prevent high-fat diet-induced obesity in mice by preventing excessive weight gain and metabolic disturbances, potentially through activating complex IV activity and preserving mitochondrial cristae structure in brown adipose tissue.
Pomegranate peel is an agro-industrial residue obtained after fruit processing with high total polyphenol (TP) content, making it an attractive by-product for its reuse. Pomegranate peel extract (PPE) and its bioactive compounds have shown positive effects on obesity models. Effects on favouring mitochondrial biogenesis and function have also been described. However, once phenolic compounds are extracted, their stability can be affected by diverse factors. Microencapsulation could improve PPE stability, allowing its incorporation into functional foods. Nevertheless, studies on the potential biological effects of PPE microparticles (MPPE) in obesity models are lacking. This study aims to evaluate the effect of MPPE on brown adipose tissue (BAT) mitochondrial structure and function and metabolic alterations related to obesity in mice fed a high-fat diet (HFD). PPE was microencapsulated by spray drying using inulin (IN) as a wall material and physically-chemically characterised. Eight-week-old male C57BL/6J mice (n 40) were randomly distributed into five groups: control diet (CD), HFD, HFD + IN, HFD + PPE (50 mg/kg per d TP) and HFD + MPPE (50 mg/kg per d TP), for 14 weeks. A glucose tolerance test and indirect calorimetry were conducted. Blood and adipose tissue samples were obtained. MPPE supplementation prevented HFD-induced body weight gain (P < 0.001), fasting glycaemia (P = 0.007) and total cholesterol rise (P = 0.001). MPPE resulted in higher BAT mitochondrial complex IV activity (P = 0.03) and prevented HFD-induced mitochondrial cristae alteration (P = 0.02). In conclusion, MPPE prevented HFD-induced excessive body weight gain and associated metabolic disturbances, potentially by activating complex IV activity and preserving mitochondrial cristae structure in BAT in mice fed with a HFD.
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