期刊
BRAIN RESEARCH
卷 1754, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.brainres.2020.147237
关键词
Insular cortex; Deep brain stimulation; Chronic pain; Rats; GABA
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2015/23522-8, 2017/25399-4, 2017/07411-7, 2018/18483-1, 2015/17136-8]
- Fundacao Faculdade de Medicina
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [159266/2017-3]
Repetitive electrical stimulation of the insular cortex can induce sustained mechanical antinociception without affecting locomotor activity. The study shows neuroplastic changes in central pain areas after repetitive ESI, with the involvement of GABAergic signaling in the effects of ESI.
The insula has emerged as a critical target for electrical stimulation since it influences pathological pain states. We investigated the effects of repetitive electrical stimulation of the insular cortex (ESI) on mechanical nociception, and general locomotor activity in rats subjected to chronic constriction injury (CCI) of the sciatic nerve. We also studied neuroplastic changes in central pain areas and the involvement of GABAergic signaling on ESI effects. CCI rats had electrodes implanted in the left agranular posterior insular cortex (pIC), and mechanical sensitivity was evaluated before and after one or five daily consecutive ESIs (15 min each, 60 Hz, 210 mu s, 1 V). Five ESIs (repetitive ESI) induced sustained mechanical antinociception from the first to the last behavioral assessment without interfering with locomotor activity. A marked increase in Fos immunoreactivity in pIC and a decrease in the anterior and mid-cingulate cortex, periaqueductal gray and hippocampus were noticed after five ESIs. The intrathecal administration of the GABA(A) receptor antagonist bicuculline methiodide reversed the stimulation-induced antinociception after five ESIs. ESI increased GAD65 levels in pIC but did not interfere with GABA, glutamate or glycine levels. No changes in GFAP immunoreactivity were found in this work. Altogether, the results indicate the efficacy of repetitive ESI for the treatment of experimental neuropathic pain and suggest a potential influence of pIC in regulating pain pathways partially through modulating GABAergic signaling.
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