期刊
BIOTECHNOLOGY JOURNAL
卷 16, 期 4, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.202000315
关键词
aldehyde; biocatalysis; carboxylic acid reductase (CAR); in vitro cofactor recycling; pharma synthon
资金
- Austrian Research Promotion Agency FFG [870897]
- TU Wien
The study introduces a novel method for aldehyde formation using carboxylic acid reductases, successfully synthesizing a pharmaceutical intermediate through a cell-free system with energy and reducing equivalents provided by auxiliary proteins.
The scarcity of practical methods for aldehyde synthesis in chemistry necessitates the development of mild, selective procedures. Carboxylic acid reductases catalyze aldehyde formation from stable carboxylic acid precursors in an aqueous solution. Carboxylic acid reductases were employed to catalyze aldehyde formation in a cell-free system with activation energy and reducing equivalents provided through auxiliary proteins for ATP and NADPH recycling. In situ product removal was used to suppress over-reduction due to background enzyme activities, and an N-protected 4-formyl-piperidine pharma synthon was prepared in 61% isolated yield. This is the first report of preparative aldehyde synthesis with carboxylic acid reductases employing crude, commercially available enzyme preparations.
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