4.6 Article

The regulatory effect of 6-TG on lncRNA-miRNA-mRNA ceRNA network in triple-negative breast cancer cell line

期刊

BIOSCIENCE REPORTS
卷 41, 期 2, 页码 -

出版社

PORTLAND PRESS LTD
DOI: 10.1042/BSR20203890

关键词

-

资金

  1. National Key R&D Program of China [2017YFA0104400]
  2. National Natural Science Foundation of China [31972874]
  3. Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) [IRT 16R32]

向作者/读者索取更多资源

Breast cancer, especially triple-negative breast cancer, is challenging to treat due to the lack of therapeutic targets. The drug thioguanine has shown antitumor effects and may potentially affect tumor pathways by down-regulating key genes. Competitive endogenous ribonucleic acids may play a role in the regulation of these pathways and could serve as potential targets for alternative breast cancer treatments.
Breast cancer is one of the most prevalent and recurring cancer types that leads to deaths in women. Triple-negative breast cancer (TNBC) is difficult to treat due to the lack of therapeutic targets. Many studies have focused on identifying drugs for use as alternative treatments for breast cancer. Thioguanine (6-TG) exerts antitumor effects in cancer. Increasing evidence has demonstrated that competitive endogenous ribonucleic acids (ceRNAs) are involved in cancer processes. However, the mechanism by which 6-TG regulates lncRNA-miRNA-mRNAs has not been elucidated. We evaluated the antitumor effect of 6-TG in MDA-MB-231 cells and comprehensively analyzed the RNA-Seq data of MDA-MB-231 cells treated with 6-TG. Our results showed that most tumor pathways were blocked by 6-TG. The hub genes were FN1, FLNA, FLNB, VCL, GSN, MYH10, ACTN4, KDR and EREG, and they were all down-regulated after 6-TG treatment. The coexpression network consisted of 18 microRNAs (miRNAs), 9 long noncoding RNAs (lncRNAs) and 20 mRNAs. Hsa-mir-16-5p and Hsa-mir-335-5p targeted the greatest number of mRNAs in the network. These molecules could bind to PAX8-AS1 and eliminate the inhibition of target mRNA expression. We showed that PAX8-AS1 is the main lncRNA affected by 6-TG and that PAX8-AS1 regulates the hub genes in tumor pathways by competitively binding with miR-16-5p and miR-335-5p.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据