Role of extracellular polymeric substances and enhanced performance for biological removal of carbonaceous organic matters and ammonia from wastewater with high salinity and low nutrient concentrations

The role of EPS in removal of carbonaceous organic matters and NI-j-N in simulated mariculture wastewater was examined at salinity of 0-3.5% in a multi-soil-layering bioreactor. Results showed that at 3.5% of salinity, the total activity of dehydrogenases (which were used to decompose carbonaceous organic matters) could be promoted by 13.2%-33.8% by EPS, increasing the removal rates of COD and NH4+-N by 13.2%-33.8% and 27.8%-42.1%, respectively. Besides, the activity of amylase in EPS was enhanced by 79.8%. However, reactions of some key enzymes such as acetate kinase and Na+K+-ATPase would not be accelerated by EPS, resulting in an inhibition of 44.3%-57.7% on energy gaining from ATP, and further inducing cytotoxicity. It was found that the glycolysis efficiency was promoted by 4.12%-59.3% in the presence of EPS, and glycolysis could also occur in EPS. Additionally, tyrosine was the main component in EPS to balance osmotic pressure.

Automated summary

In simulated mariculture wastewater, EPS was found to enhance the removal efficiency of carbonaceous organic matters and ammonia nitrogen, as well as the activity of dehydrogenases and amylase. However, EPS did not accelerate the reactions of key enzymes such as acetate kinase, leading to a decrease in ATP energy gaining and cytotoxicity. Additionally, EPS could promote glycolysis efficiency and tyrosine was identified as the main component to balance osmotic pressure.