4.8 Article

Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions

期刊

BIORESOURCE TECHNOLOGY
卷 319, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.124150

关键词

Haematococcus pluvialis; Astaxanthin; Lipids; Melatonin; Metabolomics

资金

  1. National Natural Science Foundation of China [21766012]
  2. Key Science and Technology Project of Yunnan Province [2018ZG003]
  3. Health Science and Technology Personnel Training Project of Kunming [2020-SW-28]
  4. Wang Yi Expert Workstation of Yunnan Province [2019IC050]
  5. Health Research Project of Kunming Health Commission [2020-0604-114]
  6. Science and Technology Guarantee the Development of People's Livelihood Project of Kunming [2019-1-S25318000001445]
  7. Scientific Research Foundation of Yunnan Provincial Department of Education [2020Y0084]

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The study revealed that melatonin treatment upregulated the biosynthesis of astaxanthin and lipids in Haematococcus pluvialis, affecting the accumulation process through the regulation of gene expression and metabolic pathways. The treatment may promote coproduction of astaxanthin and lipids by regulating the carbon-nitrogen balance and antioxidant system.
The effect of melatonin (MT) on the coproduction of astaxanthin and lipids was studied in Haematococcus pluvialis under inductive stress conditions. The contents of astaxanthin and lipids were enhanced by 1.78and 1.3-fold, respectively. MT treatment upregulated the transcription levels of carotenogenic, lipogenic and antioxidant system-related genes and decreased the levels of abiotic stress-induced reactive oxidative species (ROS). Further metabolomic analysis suggested that the intermediates in glycolysis and TCA cycle facilitate the accumulation of astaxanthin and lipids in algae treated with MT. Meanwhile, MT treatment upregulated the metabolite levels of the gamma-aminobutyric acid (GABA) shunt, which might regulate the carbon-nitrogen balance and the antioxidant system. After MT treatment, exogenous linoleic acid, succinate, and GABA further increased the astaxanthin content. This study may help to elucidate the specific responses to MT induction in H. pluvialis and to identify novel biomarkers that may be employed to further promote astaxanthin and lipids coproduction.

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