期刊
BIORESOURCE TECHNOLOGY
卷 319, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.124140
关键词
Cellulose hydrolysate; Microbial contamination; Lactic acid; Molasses; Clostridium beijerinckii DSM 6423
资金
- Sao Paulo Research Foundation (FAPESP) [2015/20630-4, 2016/23042-9, 2017/07390-0, 2018/23983-3]
This study demonstrates that Clostridium beijerinckii DSM 6423 is able to co-ferment first- and second-generation sugars while consuming lactic acid, resulting in high butanol yield.
Enzymatic hydrolysis of lignocellulose under industrial conditions is prone to contamination by lactic acid bacteria, and in this study, a cellulose hydrolysate produced from dilute-acid pretreated sugarcane bagasse contained 13 g/L lactic acid and was used for IBE production by Clostridium beijerinckii DSM 6423. In fermentation of the cellulose hydrolysate supplemented with sugarcane molasses for nutrients and buffering of the medium (40 g/L total sugar), 92% of the lactic acid was consumed, and the butanol yield was as high as 0.28 (7.9 g/L butanol), suggesting that lactic acid was preferentially metabolized to butanol. When the hydrolysate was mixed with a detoxified bagasse hemicellulose hydrolysate and supplemented with molasses (35 g/L total sugar), the culture was able to exhaust glucose and utilized sucrose (by 38%), xylose (31%), and lactic acid (70%). Overall, this study shows that C. beijerinckii DSM 6423 can co-ferment firstand second-generation sugars while consuming lactic acid.
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