Oxytetracycline stress reconstruct the core microbial community related to nitrogen transformation during composting

This study investigated oxytetracycline (OTC) effects on nitrogen (N) transformation and bacterial community diversity during chicken manure composting. The addition of OTC inhibited nitrifying bacteria, resulted in a decrease in the transformation of NH4+-N to NO3--N during composting, and affected in the order T3 (32.76%) > T2 (28.76%) > T1 (17.02%) > CK. The OTC could act as an inhibitor against core microbial growth, leading to a delay effect during composting. 16S rRNA sequencing was employed for the functional prediction, and results indicated the bacterial community related to N transformation reconstructed under OTC stress. The core microorganisms were changed after OTC addition, with the emergence of Bacillus and Thermobifida, which could inhibit the N transformation by network analysis. Therefore, core microorganisms could be regulated to reduce the negative of OTC impacts on N transformation and thus reduce N loss during composting.

Automated summary

This study found that oxytetracycline affects nitrogen transformation and bacterial diversity during chicken manure composting, leading to a decrease in NH4+-N to NO3--N conversion. 16S rRNA sequencing revealed a shift in core microorganisms, with the emergence of bacteria capable of inhibiting nitrogen transformation.