期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 133, 期 -, 页码 -出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2020.110977
关键词
Puerarin; Type 1 diabetes; Skeletal muscle wasting; Rats; Protein synthesis; Muscle type transformation
资金
- CAMS Initiative for Innovative Medicine (CAMS-I2M) [2016-I2M-3-007, 2017-I2M-1-010]
- National Major Research Development Program of China [2018ZX09711001-012, 2018ZX09711001-003-005, 2017YFG0112900]
- National Natural Science Foundation of China [81470159, 81770847]
The study showed that puerarin can alleviate skeletal muscle wasting in type 1 diabetes rats by promoting muscle strength and weight increase, as well as inhibiting muscle wasting.
Puerarin is an isoflavonoid extracted from Pueraria lobate with extensive pharmacological effects in traditional Chinese medicine. The evidence implicates that puerarin mitigates hyperglycemia and various relevant complications. Here, the effect of puerarin on skeletal muscle wasting induced by type 1 diabetes (T1D) was explored. Streptozotocin (STZ)-induced T1D male Sprague Dawley (SD) rats were used in this study. Muscle strength, weight and size were measured. L6 rat skeletal muscle cells were applied for in vitro study. Our results showed that eight-week oral puerarin administration (100 mg/kg) increased muscle strengths and weights accompanied by enhanced skeletal muscle cross-sectional areas in diabetic rats. Simultaneously, puerarin also reduced expressions of several muscle wasting marker genes including F-box only protein 32 (Atrogin-1) and muscle specific RING-finger 1 (Murf-1) in diabetic group both in vitro and in vivo. Transformation from type I fibers (slow muscle) to type II fibers (fast muscle) were also observed under puerarin administration in diabetic rats. Puerarin promoted Akt/mTOR while inhibited LC3/p62 signaling pathway in skeletal muscle cells. In conclusion, our study showed that puerarin mitigated skeletal muscle wasting in T1D rats and closely related with Akt/ mTOR activation and autophagy inhibition. Whether this effect in murine applies to humans remains to be determined.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据