期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 199, 期 7, 页码 2707-2716出版社
SPRINGERNATURE
DOI: 10.1007/s12011-020-02398-z
关键词
Antioxidant capacity; Epigallocatechin-3-gallate; Laying hens; Keap1-Nrf2-sMaf pathway; Vanadium
资金
- Earmarked Fund for PhD Research Start-up Fund of Henan University of Science and Technology [13480086]
In this study, epigallocatechin-3-gallate (EGCG) was found to partially alleviate the reduction of antioxidant capacity induced by dietary vanadium (V) in the liver, kidney, and ovary of laying hens through the Keap1-Nrf2-sMaf pathway. EGCG supplementation completely prevented the V-induced reductions in egg production and quality, as well as serum hormone levels, while also reversing the V-induced increases in certain biochemical markers.
This study evaluated the effect of epigallocatechin-3-gallate (EGCG) alleviating the reduction of antioxidant capacity induced by dietary vanadium (V) in the liver, kidney, and ovary of laying hens. Furthermore, Kelch-like ECH-associated protein 1(Keap1)-nuclear factor erythroid 2-related factor 2 (Nrf2)-small Maf proteins (sMaf) pathway was explored to reveal the molecular mechanism. A total of 768 40-week-old Hyline-Brown laying hens were randomly allocated to 4 groups with 8 pens per group and 24 hens per pen. The experimental groups were as follows: control (basal diet); V15, control + 15 mg/kg V; EGCG150, control + 150 mg/kg EGCG; V15 + EGCG150, V15 + 150 mg/kg EGCG. Our results revealed that dietary EGCG supplementation completely alleviated the V-induced reductions of hen-day egg production, average egg weight, Haugh unit, albumen height, eggshell strength, and eggshell thickness. Dietary EGCG supplementation completely prevented the V-induced reductions of serum follicle-stimulating hormone and luteinizing hormone levels. Besides, dietary EGCG supplementation reversed the V-induced increments of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (Cr), and uric acid (UA). In addition, dietary EGCG supplementation partially alleviated the V-induced reductions of the enzyme activities and gene expressions of superoxidative dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GSH-Px). Furthermore, dietary EGCG supplementation partially alleviated the V-induced reductions of Nrf2 and sMaf gene expressions, and the increments of Keap1 gene expression. In summary, EGCG partially alleviated V-induced reduction of antioxidant capacity through Keap1-Nrf2-sMaf pathway in the liver, kidney, and ovary of laying hens.
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