PICKLE 3.0: enriching the human meta-database with the mouse protein interactome extended via mouse-human orthology

A Summary: The PICKLE 3.0 upgrade refers to the enrichment of this human protein-protein interaction (PPI) meta-database with the mouse protein interactome. Experimental PPI data between mouse genetic entities are rather limited; however, they are substantially complemented by PPIs between mouse and human genetic entities. The relational scheme of PICKLE 3.0 has been amended to exploit the Mouse Genome Informatics mouse-human ortholog gene pair collection, enabling (i) the extension through orthology of the mouse interactome with potentially valid PPIs between mouse entities based on the experimental PPIs between mouse and human entities and (ii) the comparison between mouse and human PPI networks. Interestingly, 43.5% of the experimental mouse PPIs lacks a corresponding by orthology PPI in human, an inconsistency in need of further investigation. Overall, as primary mouse PPI datasets show a considerably limited overlap, PICKLE 3.0 provides a unique comprehensive representation of the mouse protein interactome.

Automated summary

To enrich the human protein-protein interaction (PPI) meta-database, the PICKLE 3.0 upgrade incorporates mouse protein interactome data, utilizing ortholog gene pairs to extend the mouse interactome and compare mouse and human PPI networks. Despite 43.5% of experimental mouse PPIs lacking corresponding human orthologs, PICKLE 3.0 provides a comprehensive representation of the mouse protein interactome.