期刊
BIOELECTROCHEMISTRY
卷 139, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2021.107742
关键词
Cysteamine; sPD-L1 protein; Gold electrode modification; Cyclic voltammetry (CV); Electrochemical impedance spectroscopy (EIS)
资金
- National Science Centre, Poland (NCN) [2020/37/B/ST7/03262, 2017/25/B/NZ1/00827, 2014/12/W/NZ1/00457]
- Polpharma Scientific Foundation [3/XVIII/2019]
- TEAM program of the Foundation for Polish Science [POIR.04.04.0000420F/1700]
- European Union under the European Regional Development Fund
This study successfully demonstrates the modification of a gold electrode with BMS-8 compound for interaction with PD-L1, and further investigates the selectivity of the electrode for other proteins. The work provides a new approach for electrochemical detection of immune checkpoint proteins.
This work describes the modification of a gold electrode with the BMS-8 compound that interacts with the Programmed Death-Ligand 1 (PD-L1), an immune checkpoint protein. The results show that we can confirm the presence of the sPD-L1 in the concentration range of 10(-18) to 10(-8) M using electrochemical impedance spectroscopy (EIS) with a limit of detection (LOD) of 1.87 x 10(-14) M for PD-L1 (S/N = 3.3) and at a concentration of 10(-14) M via cyclic voltammetry (CV). Additionally, high-resolution X-ray photoelectron spectroscopy (XPS), contact angle, and surface free energy measurements were applied to confirm the functionalization of the electrode. We investigated the selectivity of the electrode for other proteins: Programmed Death-1 (PD-1), cluster of differentiation 160 (CD160), and B- and T-lymphocyte attenuator (BTLA) at concentrations of 10(-8) M. Differentiation between PD-L1 and PD-1 was achieved based on the analysis of the capacitance effect frequency dispersion at the surface of the modified Au electrode with BMS-8 after incubation at various concentrations of PD-L1 and PD-1 proteins in the range of 10(-18) to 10(-8) M. Significant differences were observed in the heterogeneity of PD-L1 and PD-1. The results of the quasi-capacitance studies demonstrate that BMS-8 strongly and specifically interacts with the PD-L1 protein. (C) 2021 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据