期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
卷 1863, 期 1, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbamem.2020.183486
关键词
Candida albicans; Potassium transport; Trk1; Acu1; Hak1; Heterologous expression
资金
- XXII Plan Propio Investigacion, University of Cordoba
- MICIU [RTI2018-097935-B-I00]
- Ministry of Education, Youth and Sports of CR [LQ1604]
- project BIOCEV [CZ.1.05/1.1.00/02.0109]
- project Inter-COST [LTC20006]
Wild-type cells of Candida albicans can grow at low potassium concentrations due to the presence of three different types of K+ transporters in their plasma membrane, which are regulated differently in response to saline stress, pH alterations or K+ starvation. Trk1 is the house-keeping transporter, while Acu1 and Hak1 dominate under K+ limiting conditions. Expression of these transporters in Saccharomyces cerevisiae also affects other physiological characteristics, with CaTrk1 being the most effective in maintaining these parameters close to wild-type levels.
Wild-type cells of Candida albicans, the most common human fungal pathogen, are able to grow at very low micromolar concentrations of potassium in the external milieu. One of the reasons behind that behaviour is the existence of three different types of K+ transporters in their plasma membrane: Trk1, Acu1 and Hak1. This work shows that the transporters are very differently regulated at the transcriptional level upon exposure to saline stress, pH alterations or K+ starvation. We propose that different transporters take the lead in the diverse environmental conditions, Trk1 being the house-keeping one, and Acu1/Hak1 dominating upon K+ limiting conditions. Heterologous expression of the genes coding for the three transporters in a Saccharomyces cerevisiae strain lacking its endogenous potassium transporters showed that all of them mediated cation transport but with very different efficiencies. Moreover, expression of the transporters in S. cerevisiae also affected other physiological characteristics such as sodium and lithium tolerance, membrane potential or intracellular pH, being, in general, CaTrk1 the most effective in keeping these parameters close to the usual wild-type physiological levels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据