期刊
BIOCHEMICAL ENGINEERING JOURNAL
卷 165, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bej.2020.107798
关键词
Fucoidan; Sargassum siliquosum; Purification; Anti-lipogenesis
资金
- Ministry of Science and Technology, Taiwan [MOST 107-2221-E-006-112-MY3, 108-2621-M-006-020-, 109-3116-F-006-016-CC1]
Fucoidans, sulfated polysaccharides of marine origin, were extracted from brown macroalgae using microwave-assisted and ultrasound-assisted extraction methods. The purified fucoidan product showed nearly 84.0% purity and exhibited anti-lipogenesis activity with a 28.9% decrease in lipid synthesis compared to the control.
Fucoidans are sulfated polysaccharides of marine origin and they have distinguished biological applications due to their anti-viral, anti-inflammatory, anti-angiogenic, and anti-tumorigenic potential. In this study, fucoidan was extracted from a brown macroalga (Sargassum siliquosum) using microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE). MAE under the condition of 750 W, 10 min, and a liquid/solid ratio of 15 mL/g, showed higher extraction efficiency with a total sugar yield of 6.94% per dry weight. The crude fucoidan obtained with ethanol precipitation was further purified by isoelectric point precipitation and calcium chloride addition (for protein and uronic acid removal), followed by anion-exchange chromatography (AEC) and dialysis. The purity of the polysaccharides content of crude fucoidan increased from 33.3-64.5% with a total sugar recovery of 50.3%. Based on the sugar and sulfate group (the major components of fucoidan), the purity of the final sulfated polysaccharides product is nearly 84.0%, and this purified fucoidan product was found to display biological activities linked to anti-lipogenesis with 28.9% decreased lipid synthesis in contrast to the free-fatty-acid-induced control.
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