期刊
AUTOPHAGY
卷 17, 期 11, 页码 3408-3423出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15548627.2021.1874134
关键词
Antiretroviral therapy; elite controllers; FAO; HIV-1; IL21; lipophagy; polyfunctionality
类别
资金
- Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada
- Fonds de Recherche du Quebec - Sante
Autophagy/lipophagy can be considered as a therapeutic tool to enhance functional antiviral CD8 T-cell responses, providing new strategies to improve HIV-1-specific CD8A T-cell function. IL21 treatment is effective in rescuing antiviral CD8A T-cell immunity from ART, with the enhanced degradation of endogenous lipids via autophagy playing a critical role in this process.
Although macroautophagy/autophagy has been proposed as a critical defense mechanism against HIV-1 by targeting viral components for degradation, its contribution as a catabolic process in providing optimal anti-HIV-1 immunity has never been addressed. The failure to restore proper antiviral CD8A/CD8 T-cell immunity, especially against HIV-1, is still the major limitation of current antiretroviral therapies. Consequently, it is of clinical imperative to provide new strategies to enhance the function of HIV-1-specific CD8A T-cells in patients under antiretroviral treatments (ART). Here, we investigated whether targeting autophagy activity could be an optional solution to make this possible. Our data show that, after both polyclonal and HIV-1-specific activation, CD8A T-cells from ART displayed reduced autophagy-dependent degradation of lysosomal contents when compared to naturally HIV-1 protected elite controllers (EC). We further confirmed in EC, by using specific BECN1 gene silencing and lysosomal inhibitors, the critical role of active autophagy in superior CD8A T-cell protection against HIV-1. More importantly, we found that an IL21 treatment was effective in rescuing the antiviral CD8A T-cell immunity from ART in an autophagy-dependent manner. Finally, we established that IL21-dependent rescue occurred due to the enhanced degradation of endogenous lipids via autophagy, referred to as lipophagy, which fueled the cellular rates of mitochondrial beta-oxidation. In summary, our data show that autophagy/lipophagy can be considered as a therapeutic tool to elicit functional antiviral CD8 T-cell responses. Our results also provide additional insights toward the development of improved T-cell-based prevention and cure strategies against HIV-1.
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