Nerve guidance conduit promoted peripheral nerve regeneration in rats

Nerve growth factor (NGF) is important for peripheral nerve regeneration. However, its short half-life and rapid diffusion in body fluids limit its clinical efficacy. Collagen has favorable biocompatibility and biodegradability, and weak immunogenicity. Because it possesses an NGF binding domain, we cross-linked heparin to collagen tubes to construct nerve guidance conduits for delivering NGF. The conduits were implanted to bridge a facial nerve defect in rats. Histological and functional analyses were performed to assess the effect of the nerve guidance conduit on facial nerve regeneration. Heparin enhanced the binding of NGF to collagen while retaining its bioactivity. Also, the nerve guidance conduit significantly promoted axonal growth and Schwan cell proliferation at 12 weeks after surgery. The nerve regeneration and functional recovery outcomes using the nerve guidance conduit were similar to those of autologous nerve grafting. Therefore, the nerve guidance conduit may promote safer nerve regeneration.

Automated summary

Heparin-crosslinked collagen nerve guidance conduits successfully delivered NGF and promoted nerve regeneration at the site of facial nerve defect in rats. The conduits enhanced axonal growth and Schwann cell proliferation, with similar nerve regeneration and functional recovery outcomes as autologous nerve grafting. The nerve guidance conduit may provide a safer approach for nerve regeneration.