4.4 Article

A novel emaravirus comprising five RNA segments is associated with ringspot disease in oak

期刊

ARCHIVES OF VIROLOGY
卷 166, 期 3, 页码 987-990

出版社

SPRINGER WIEN
DOI: 10.1007/s00705-021-04955-w

关键词

-

类别

资金

  1. FAZIT-STIFTUNG Gemeinnutzige Verlagsgesellschaft mbH
  2. German Research Foundation [Bu890/27-1]
  3. European Cooperation in Science and Technology [FA1407]

向作者/读者索取更多资源

This study reports the discovery of a novel virus in ringspot-diseased common oak trees and successfully determined the complete genome sequence. By using high-throughput sequencing and RT-PCR techniques, a new virus belonging to the genus Emaravirus was identified, affecting oak trees in Germany and Scandinavia.
We report the complete nucleotide sequence of the genome of a novel virus in ringspot-diseased common oak (Quercus robur L.). The newly identified pathogen is associated with leaf symptoms such as mottle, chlorotic spots and ringspots on diseased trees. High-throughput sequencing (HTS, Illumina RNASeq) was used to explore the virome of a ringspot-diseased oak that had chlorotic ringspots of suspected viral origin on leaves for several years. Bioinformatic analysis of the HTS dataset followed by RT-PCR enabled us to determine complete sequences of four RNA genome segments of a novel virus. These sequences showed high similarity to members of the genus Emaravirus, which includes segmented negative-stranded RNA viruses of economic importance. To verify the ends of each RNA, we conducted rapid amplification of cDNA ends (RACE). We identified an additional genome segment (RNA 5) by RT-PCR using a genus-specific primer (PDAP213) to the conserved 3 ' and 5 ' termini in order to amplify full-length genome segments. RNA 5 encodes a 21-kDa protein that is homologous to the silencing suppressor P8 of High Plains wheat mosaic virus. The five viral RNAs were consistently detected by RT-PCR in ringspot-diseased oaks in Germany, Sweden, and Norway. We conclude that the virus represents a new member of the genus Emaravirus affecting oaks in Germany and in Scandinavia, and we propose the name common oak ringspot-associated emaravirus (CORaV).

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据