期刊
ARCHIVES OF MEDICAL SCIENCE
卷 17, 期 1, 页码 127-134出版社
TERMEDIA PUBLISHING HOUSE LTD
DOI: 10.5114/aoms.2019.85404
关键词
ovarian cancer; apoptosis; microRNA; proliferation
资金
- Natural science Fund of Science and Technology Department, Jilin [20180101010JC]
The research demonstrated that miRNA-101 is down-regulated in ovarian cancer cells and its over-expression can suppress the growth of ovarian cancer cells by inducing apoptosis and cell cycle arrest, potentially through regulating molecules like PTEN, PI3K, and AKT.
Introduction: Ovarian cancer is the most frequent cause of gynecological cancer related mortality in woman. This study was designed to investigate the role and therapeutic potential of miRNA-101 in ovarian cancer. Material and methods: Expression analysis was carried out by real-time quantitative polymerase chain reaction. Transfections were performed with the help of Lipofectamine 2000 reagent. AO/EB and annexin V/PI staining was used to detect apoptosis and flow cytometry was used for cell cycle analysis. Western blotting was employed for cell cycle analysis. Results: It was found that miRNA-101 was significantly down-regulated in ovarian cancer cells. The over-expression of miRNA-101 causes a significant decrease in the viability of ovarian cancer cells via the initiation of apoptosis and sub-G1 arrest of OVACAR-3 cells. It was indicated that PTEN was the potential target of miRNA-101 in OVACAR-3 cells. There was 4.5-fold up-regulation of PTEN expression in ovarian cancer cell lines and the over-expression of miRNA-101 in OVACAR-3 cells resulted in the down-regulation of PTEN expression. The inhibition of PTEN in the OVACAR-3 cells arrested the proliferation of these cells. The over-expression of miRNA-101 causes significant down-regulation in PI3K and AKT expression of OVACAR-3 cells. Conclusions: It can be concluded that miRNA-101 acts as a tumor suppressor which may be beneficial in the treatment of ovarian cancer.
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