期刊
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
卷 127, 期 1, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00339-020-04171-4
关键词
TOV6 aptamer; Stress-induced phosphoprotein 1; Multimodal fluorescence; magnetic resonance imaging nanoprobe; Human ovarian cancer
资金
- Isfahan University of Medical Sciences [396461]
- University of Isfahan Research Council
The study presents a multifunctional aptamer nanoprobe for fluorescence and magnetic resonance targeted imaging of human ovarian cancer cells, demonstrating high cytotoxic effects and tumor-targeting capabilities, making it a potential contrast agent for MRI and fluorescent probe for microscopic imaging.
A multifunctional-aptamer (APT) nanoprobe, TOV6 APT-superparamagnetic iron oxide nanoparticle-carbon dots (APT-SPION-CDs), for fluorescence and magnetic resonance targeted imaging (FI/MRI) of human ovarian cancer cells (OVCAR-3) is introduced. The SPION-CDs were synthesized and conjugated with TOV6 APT recognizing the stress-induced phosphoprotein 1 (STIP1) overexpressed on OVCAR-3 cells. The capability of the nanoprobe as a contrast agent for MRI and simultaneously as a fluorescent probe for fluorescence microscopy was studied. TOV6 APTs were adsorbed on SPION-CDs and were confirmed with Fourier transform infrared spectrometer. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed higher cytotoxic effects for APT-SPION-CDs compared to SPION-CDs on STIP1-negative HeLa cells. But on OVCAR-3, as STIP1-positive cells, the cytotoxic effect was negligible. The fluorescence microscopic images confirmed that APT-SPION-CDs specifically targeted ovarian cancer cells due to the tumor-targeting effect of TOV6 APT. High spin-spin relaxivity value (r(2)=308.5 mM(-1) s(-1)) and the signal enhancement in T-2-weighted MRIs confirmed the capability of the nanoprobe as a T-2-based contrast agent. In vitro cellular uptake and signal enhancement of this multimodal FI/MRI nanoprobe demonstrated the potential application of APT-SPION-CDs as a contrast agent for MRI and as a fluorescent probe for fluorescence microscopic imaging.
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