期刊
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷 105, 期 3, 页码 1237-1251出版社
SPRINGER
DOI: 10.1007/s00253-020-11027-7
关键词
Dicephalic cationic surfactants; Cell's membrane; ROS; Saccharomyces cerevisiae
资金
- Polish Ministry of Science and Higher Education
The research found that the mechanism of perforation of cell membranes commonly associated with surfactants does not apply to cationic dicephalic surfactants, which instead act by increasing cell membrane permeability, leading to lipid droplet aggregation and morphological changes in fungal cells. Additionally, these compounds induce oxidative stress within cells, including increased production of superoxide anion radicals and mitochondrial oxidative stress.
Our research aimed to expand the knowledge of relationships between the structure of multifunctional cationic dicephalic surfactants with a labile linker-N,N-bis[3,3-(dimethylamine)propyl]alkylamide dihydrochlorides and N,N-bis[3,3-(trimethylammonio)propyl]alkylamide dibromides (alkyl: n-C9H19, n-C11H23, n-C13H27, n-C15H31)-and their possible mechanism of action on fungal cells using the model organism Saccharomyces cerevisiae. General studies performed on surfactants suggest that in most cases, their main mechanism of action is based on perforation of the cell membranes and cell disruption. Experiments carried out in this work with cationic dicephalic surfactants seem to modify our understanding of this issue. It was found that the investigated compounds did not cause perforation of the cell membrane and could only interact with it, increasing its permeability. The surfactants tested can probably penetrate inside the cells, causing numerous morphological changes, and contribute to disorders in the lipid metabolism of the cell resulting in the formation of lipid droplet aggregates. This research also showed that the compounds cause severe oxidative stress within the cells studied, including increased production of superoxide anion radicals and mitochondrial oxidative stress. Dicephalic cationic surfactants due to their biodegradability do not accumulate in the environment and in the future may be used as effective antifungal compounds in industry as well as medicine, which will be environmentally friendly.
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