期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 193, 期 5, 页码 1545-1557出版社
SPRINGER
DOI: 10.1007/s12010-021-03508-w
关键词
Inonotus obliquus; Polysaccharide; alpha-Glucosidase; Birch sawdust; Pretreatment
资金
- National Natural Science Foundation of China [21576282]
This study demonstrated that alkali treatment of birch sawdust was the most effective method in promoting the production of active polysaccharides by Inonotus obliquus. Through structural characterization and analysis, it was shown that alkali treatment destroyed the lignin structure of birch sawdust and exposed the cellulose for better utilization.
This study examined the effects of different pretreatments of birch sawdust on the production and activity of polysaccharides by Inonotus obliquus, and in order to explore the mechanism, structural characterization and analysis were carried out. The result clearly indicated that alkali treatment, ozone treatment, and alkali combined with ozone treatment of birch sawdust could be all helpful for the production of active polysaccharide by I. obliquus. Among four pretreatment groups, birch sawdust treated with alkali showed the highest increase in the exo-polysaccharide content (39.90%) and the inhibition rate of alpha-glucosidase (80.78%) within 11 days by the mycelium of I. obliquus through deep fermentation, in comparison to water-washed birch sawdust. Through a single-factor analysis and orthogonal experimental design, the optimum alkali treatment condition was as follows: NaOH concentration 1%, temperature 60 degrees C, and time 3 h. Moreover, the structural characteristics of pretreated birch sawdust with the optimum alkali treatment condition before and after fermentation by the mycelium of I. obliquus was performed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electronic microscopy. The results showed that alkali treatment destroyed the lignin structure of birch sawdust, exposed the cellulose in the amorphous area, reduced the crystallinity of lignocellulose, and damaged the surface structure of birch sawdust, which had a further damage and a greater degradation degree of birch sawdust after fermentation, indicating that alkali pretreatment was beneficial for utilization of birch sawdust by I. obliquus.
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