Description of Cohnella zeiphila sp. nov., a bacterium isolated from maize callus cultures

A Gram-stain positive, aerobic, motile, rod-shaped bacterium designated as strain CBP-2801(T) was isolated as a contaminant from a culture containing maize callus in Peoria, Illinois, United States. The strain is unique relative to other Cohnella species due to its slow growth and reduced number of sole carbon sources. Phylogenetic analysis using 16S rRNA indicated that strain CBP-2801(T) is a Cohnella bacterium and showed the highest similarity to Cohnella xylanilytica (96.8%). Genome-based phylogeny and genomic comparisons based on average nucleotide identity confirmed the strain to be a novel species of Cohnella. Growth occurs at 15-45 degrees C (optimum 40 degrees C), pH 5-7 (optimum pH 6) and with 0-1% NaCl. The predominant fatty acids are anteiso-15:0 and 18:1 omega 6c. Genome mining for secondary metabolites identified a putative biosynthetic cluster that encodes for a novel lasso peptide. In addition, this study contributes five new genome assemblies of type strains of Cohnella species, a genus with less than 30% of the type strains sequenced. The DNA G + C content is 58.7 mol %. Based on the phenotypic, phylogenetic and biochemical data strain CBP-2801(T) represents a novel species, for which the name Cohnella zeiphila sp. nov. is proposed. The type strain is CBP-2801(T) (= DSM 111598 = ATCC TSD-230).

Automated summary

A novel strain of Cohnella bacterium named Cohnella zeiphila sp. nov. was isolated from a culture of maize callus in Peoria, Illinois, United States. Through phenotypic, phylogenetic, and genomic analysis, the strain was confirmed to be unique within the Cohnella genus. This study also presents genome data for five type strains of Cohnella species, contributing to a better understanding of this genus.