4.7 Article

Characterization of a Plasmid-Encoded Resistance-Nodulation Division Efflux Pump in Klebsiella pneumoniae and Klebsiella quasipneumoniae from Patients in China

期刊

出版社

AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.02075-20

关键词

Klebsiella spp; efflux pump; tmexCD1-toprJ1; tigecycline; antimicrobial resistance

资金

  1. National Natural Science Foundation of China [81722030, 81830103, 81902123]
  2. National Key Research and Development Program [2017ZX10302301]
  3. Guangdong Natural Science Foundation [2017A030306012]
  4. Project of High-Level Health Teams of Zhuhai in 2018 (The Innovation Team for Antimicrobial Resistance and Clinical Infection)
  5. 111 Project [B12003]
  6. Open Project of the Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education [2020kfkt04, 2020kfkt07]
  7. China Postdoctoral Science Foundation [2019M653192]
  8. Science, Technology, & Innovation Commission of Shenzhen Municipality [JCYJ20190807151601699]

向作者/读者索取更多资源

The study identified two MDR Klebsiella pneumoniae isolates carrying mcr-1 from patients with urinary tract infections, and one MDR Klebsiella quasipneumoniae isolate carrying tmexCD1-toprJ1 from a patient with bloodstream infection. The addition of an efflux pump inhibitor significantly reduced tigecycline resistance in these isolates. Additionally, a plasmid carrying tmexCD1-toprJ1 was successfully transferred via conjugation from K. quasipneumoniae to Escherichia coli J53 for further analysis.
Two multidrug-resistant (MDR) mcr-1-harboring Klebsiella pneumoniae isolates from patients with urinary tract infections and one MDR Klebsiella quasipneumoniae isolate from a patient with bloodstream infection were identified to carry tmexCD1-toprJ1. The addition of the efflux pump inhibitor reduced the tigecycline MIC against all three isolates by 8- to 16-fold. pKQBSI104-1 was transferred from K. quasipneumoniae to Escherichia coli J53 via conjugation. The tmexCD1-toprJ1-carrying plasmids pKP15ZE495-1 (102,569 bp) and pKQBSI104-1 (121,996 bp) were completely sequenced and analyzed.

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