期刊
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
卷 65, 期 2, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.02075-20
关键词
Klebsiella spp; efflux pump; tmexCD1-toprJ1; tigecycline; antimicrobial resistance
资金
- National Natural Science Foundation of China [81722030, 81830103, 81902123]
- National Key Research and Development Program [2017ZX10302301]
- Guangdong Natural Science Foundation [2017A030306012]
- Project of High-Level Health Teams of Zhuhai in 2018 (The Innovation Team for Antimicrobial Resistance and Clinical Infection)
- 111 Project [B12003]
- Open Project of the Key Laboratory of Tropical Disease Control (Sun Yat-sen University), the Ministry of Education [2020kfkt04, 2020kfkt07]
- China Postdoctoral Science Foundation [2019M653192]
- Science, Technology, & Innovation Commission of Shenzhen Municipality [JCYJ20190807151601699]
The study identified two MDR Klebsiella pneumoniae isolates carrying mcr-1 from patients with urinary tract infections, and one MDR Klebsiella quasipneumoniae isolate carrying tmexCD1-toprJ1 from a patient with bloodstream infection. The addition of an efflux pump inhibitor significantly reduced tigecycline resistance in these isolates. Additionally, a plasmid carrying tmexCD1-toprJ1 was successfully transferred via conjugation from K. quasipneumoniae to Escherichia coli J53 for further analysis.
Two multidrug-resistant (MDR) mcr-1-harboring Klebsiella pneumoniae isolates from patients with urinary tract infections and one MDR Klebsiella quasipneumoniae isolate from a patient with bloodstream infection were identified to carry tmexCD1-toprJ1. The addition of the efflux pump inhibitor reduced the tigecycline MIC against all three isolates by 8- to 16-fold. pKQBSI104-1 was transferred from K. quasipneumoniae to Escherichia coli J53 via conjugation. The tmexCD1-toprJ1-carrying plasmids pKP15ZE495-1 (102,569 bp) and pKQBSI104-1 (121,996 bp) were completely sequenced and analyzed.
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