Effects of methyl farnesoate on Kruppel homolog 1 (Kr-h1) during vitellogenesis in the Chinese mitten crab (Eriocheir sinensis)

Methyl farnesoate (MF), a de-epoxidized form of juvenile hormone (JH) III in insects, may regulate developmental processes such as reproduction and ovarian maturation in crustaceans. Kruppel homolog 1 (Kr-h1) is a target response gene for the methoprene-tolerant (Met) protein that is a component of the JH signaling pathway in insects. In the present study, Es-Kr-h1 was cloned from E. sinensis and characterized to ascertain whether JH/MF signaling in insects is conserved in crustaceans. The findings with molecular structure analysis indicated Es-Kr-h1 contains seven zinc finger motifs (Zn2-Zn8) commonly conserved in other crustaceans, but the Zn1 motif was not detected to be present. The PCR results indicated that relative abundance of EsKr-h1 mRNA transcript in the hepatopancreas was greatest in the Stage II, followed by the Stage IV ovarian developmental categories. The relative abundance of Es-Kr-h1 mRNA transcript in vitro was greater after MF addition to the hepatopancreas, however, not the ovarian tissues. The results from in vivo and eyestalk ablation experiments indicated the relative abundance of Es-Kr-h1 mRNA transcript was greater after MF treatment and bilateral eyestalk removal in the hepatopancreas, however, not ovarian tissues. Notably, there were effects of MF on relative abundance of Es-Kr-h1 mRNA transcript pattern. The Es-Kr-h1 protein, therefore, may be involved in MF-mediated vitellogenesis resulting from the response to Es-Met in E. sinensis, and the JH/MF signaling pathway is potentially conserved in crustaceans.

Automated summary

The study explored the role of Es-Kr-h1 gene in crustacean development, suggesting its involvement in ovarian maturation and the JH/MF signaling pathway in E. sinensis. The results also indicated potential conservation of these pathways in crustaceans.