期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 9, 页码 4907-4914出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202012171
关键词
bioelectrochemistry; electrochemiluminescence; electrochemistry; microscopy; single-cell studies
资金
- National Natural Science Foundation of China [21974065, 21834004, 21904063]
- Excellent Research Program of Nanjing University [ZYJH004]
- Natural Science Foundation of Jiangsu Province [BK20190279, BK20200059]
- Fundamental Research Funds for the Central Universities [020514380173, 021314380151]
- Washington State University Start-up Fund
The bio-coreactant-enhanced electrochemiluminescence (ECL) microscopy allows for ECL imaging of intracellular structure and dynamic transport by using Ru(bpy)(3)(2+) and intracellular biomolecules. The research reveals the universal edge effect of cellular electroporation, as well as the dynamic transport of Ru(bpy)(3)(3+) in different cellular compartments and its correlation with the actin cytoskeleton.
A bio-coreactant-enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)(3)(2+) as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via a catalytic route. Accordingly, intracellular structures are identified without using multiple labels, and autophagy involving DNA oxidative damage is detected using nuclear ECL signals. A time-resolved image sequence discloses the universal edge effect of cellular electroporation due to the influence of the geometric properties of cell membranes on the induced transmembrane voltage. The dynamic transport of Ru(bpy)(3)(3+) in the different cellular compartments unveils the heterogeneous intracellular diffusivity correlating with the actin cytoskeleton. In addition to single-cell studies, the bio-coreactant-enhanced ECL microscopy is used to image a slice of a mouse liver and a colony of Shewanella oneidensis MR-1.
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