期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 9, 页码 4782-4788出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202012444
关键词
biosensors; DNA; enzymes; fluorescence; molecular recognition
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC) grant [STPGP 494302]
LP1 is an RNA-cleaving fluorogenic DNAzyme that is reactive with multiple infectious isolates of L. pneumophila, capable of generating a detectable signal for bacterial detection. It holds potential for the development of biosensors to monitor the contamination of L. pneumophila in exposure sources such as cooling towers.
Legionella pneumophila is a deadly bacterial pathogen that has caused numerous Legionnaires' disease outbreaks, where cooling towers were the most common source of exposure. Bacterial culturing is used for L. pneumophila detection, but this method takes approximately 10 days to complete. In this work, an RNA-cleaving fluorogenic DNAzyme, named LP1, was isolated. Extensive characterization revealed that LP1 is reactive with multiple infectious isolates of L. pneumophila but inactive with 25 other common bacterial species. LP1 is likely activated by a protein target, capable of generating a detectable signal in the presence of as few as 10 colony-forming units of L. pneumophila, and able to maintain its activity in cooling tower water from diverse sources. Given that similar DNAzymes have been incorporated into many sensitive assays for bacterial detection, LP1 holds the potential for the development of biosensors for monitoring the contamination of L. pneumophila in exposure sources.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据