期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 10, 页码 5421-5428出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202014002
关键词
biocatalysis; metal-organic frameworks; metal-phenolic networks; multienzyme; porous microreactors
资金
- Australia National Health and Medical Research Council (NHMRC) [GNT1163786]
- Australian Research Council (ARC) [DP210100422, DP190101008, DP150104485]
- Scientia program at UNSW
Hierarchically porous MOFs were constructed by controlled structural etching to enhance multienzyme and cofactor-dependent enzyme biocatalysis, which provided sufficient space for enzymes to reorientate and spread within MOFs and effectively accelerate the diffusion rate of reactants and intermediates, showing outstanding tolerance to inhospitable surroundings and recyclability.
Metal-organic frameworks (MOFs) have recently emerged as excellent hosting matrices for enzyme immobilization, offering superior physical and chemical protection for biocatalytic reactions. However, for multienzyme and cofactor-dependent biocatalysis, the subtle orchestration of enzymes and cofactors is largely disrupted upon immobilizing in the rigid crystalline MOF network, which leads to a much reduced biocatalytic efficiency. Herein, we constructed hierarchically porous MOFs by controlled structural etching to enhance multienzyme and cofactor-dependent enzyme biocatalysis. The expanded size of the pores can provide sufficient space for accommodated enzymes to reorientate and spread within MOFs in their lower surface energy state as well as to decrease the inherent barriers to accelerate the diffusion rate of reactants and intermediates. Moreover, the developed hierarchically porous MOFs demonstrated outstanding tolerance to inhospitable surroundings and recyclability.
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