Dried Blood Spot Self-Sampling with Automated Capillary Electrophoresis Processing for Clinical Analysis

A simple and convenient concept of blood sampling followed by a fully automated analysis is presented. A disposable sampling kit is used for accurate self-sampling of capillary blood from a finger prick. A high-throughput blood sampling is thus enabled, which is essential in many clinical assays and considerably improves life quality and comfort of involved subjects. The collected blood samples are mailed to a laboratory for a fully automated dried blood spot (DBS) processing and analysis, which are performed with a commercial capillary electrophoresis instrument. Quantitative results are obtained within 20 min from the DBS delivery to the laboratory. The presented concept is exemplified by the determination of warfarin blood concentrations and demonstrates excellent analytical performance. Moreover, this concept is generally applicable to a wide range of endogenous and exogenous blood compounds and represents a novel and attractive analytical tool for personalized health monitoring.

Automated summary

This study introduces a simple and convenient concept of automated blood sampling and analysis, allowing for accurate collection of blood samples and automated processing and analysis in a laboratory setting. Using warfarin blood concentration determination as an example, the concept demonstrates excellent analytical performance and wide applicability to various types of blood compounds.