期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 60, 期 10, 页码 5429-5435出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202013904
关键词
CRISPR; Cas9 genome editing; hierarchical self-assembly; protein delivery; supramolecular nanoparticles
资金
- National Key Research and Development Program of China [2017YFA0208100, 2016YFA0200104]
- National Science Foundation of China [21778056, 22077125, 21790390, 21790391]
The hierarchical self-assembly of metal-organic cages into supramolecular nanoparticles allows for efficient encapsulation of proteins for intracellular delivery and potential therapeutic applications, such as CRISPR/Cas9 genome editing.
Hierarchical self-assembly (HAS) is a powerful approach to create supramolecular nanostructures for biomedical applications. This potency, however, is generally challenged by the difficulty of controlling the HAS of biomacromolecules and the functionality of resulted HAS nanostructures. Herein, we report a modular approach for controlling the HAS of discrete metal-organic cages (MOC) into supramolecular nanoparticles, and its potential for intracellular protein delivery and cell-fate specification. The hierarchical coordination-driven self-assembly of adamantane-functionalized M12L24 MOC (Ada-MOC) and the host-guest interaction of Ada-MOC with beta-cyclodextrin-conjugated polyethylenimine (PEI-beta CD) afford supramolecular nanoparticles in a controllable manner. HAS maintains high efficiency and orthogonality in the presence of protein, enabling the encapsulation of protein into the nanoparticles for intracellular protein delivery for therapeutic application and CRISPR/Cas9 genome editing.
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