期刊
ANDROLOGIA
卷 53, 期 1, 页码 -出版社
WILEY
DOI: 10.1111/and.13903
关键词
antioxidants; infertility; Moringa oleifera; reactive oxygen species; spermatozoa
类别
资金
- National Research Foundation of South Africa [TTK170426228930]
- University of Limpopo
The study found that the aqueous leaf extract of Moringa oleifera has antioxidant properties that can reduce sperm reactive oxygen species generation, DNA fragmentation, and acrosome reaction, while maintaining sperm capacitation and vitality. Further research is needed to confirm the impact of Moringa oleifera leaf extract on fertility potential.
The effects of aqueous leaf extract of Moringa oleifera (MO) on human sperm functions and integrity was studied in vitro. Semen was obtained by masturbation after 3-5 days' abstinence from 34 healthy donors in Western Cape, South Africa. Liquefied semen was washed in human tubular fluid supplemented with 1% bovine serum albumin (HTF-BSA;1:5) with 10 min centrifugation at 300 g. Sperm suspensions were subsequently incubated with MO extract (0.625, 6.25, 62.5 and 625 mu g/ml) for 1 hr, where HTF-BSA served as control. Sperm motility, vitality, DNA fragmentation, reactive oxygen species production, mitochondrial membrane potential, capacitation and acrosome reaction were assessed. Sperm motility, vitality, mitochondrial membrane potential and capacitation remained unchanged (p > .05). A dose-dependent decrease in sperm reactive oxygen species production (p < .0001), DNA fragmentation (p < .0001) and acrosome reaction (p < .001) was observed. An increase in the percentage of non-capacitated sperm (p < .01) was noted at 625 mu g/ml. The antioxidant properties of MO actively maintained basic sperm functions, inhibited excess sperm free superoxide production and preserved acrosome reaction and DNA integrity. Further studies are needed to confirm the effect of aqueous MO leaf extract on fertility potential.
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