期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 413, 期 4, 页码 1073-1080出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-020-03067-9
关键词
Aptasensor; Potentiometric measurement; Carcinoembryonic antigen; Nanometer-sized CaCO3; Ion-selective electrode
资金
- National Natural Science Foundation of China [81703750]
Phenylboronic acid-functionalized nanometer-sized CaCO3 particles (PBA-CaCO3) were designed and utilized for detecting CEA glycoprotein through a boronate ester bond with a portable Ca2+ ion-selective electrode (Ca-ISE). The synthesized PBA-CaCO3 exhibited good conjugation properties for CEA glycoprotein, enabling sensitive detection of CEA at concentrations as low as 7.3 pg mL(-1) under optimal conditions. Additionally, the Ca-ISE-based aptasensing system can be easily extended to detect other disease-related glycoproteins by controlling the corresponding aptamer.
Phenylboronic acid-functionalized nanometer-sized CaCO3 particles (PBA-CaCO3) were designed to determine the carcinoembryonic antigen (CEA) glycoprotein with a portable Ca2+ ion-selective electrode (Ca-ISE) through a typical boronate ester bond. CaCO3 nanospheres were conjugated to 3-aminophenylboronic acid by amine-epoxy reaction, whereas target CEA was captured into the aptasensing interface by the immobilized thiolated aptamer on gold substrate. Upon PBA-CaCO3 introduction, 3-aminophenylboronic acid labeled to CaCO3 microsphere specifically recognized with CEA glycoprotein based on sugar-boronic acid interaction to form a sandwiched complex. The carried CaCO3 was dissolved under acidic conditions to release Ca2+ ion with a portable Ca-ISE readout. Thanks to the specific boronate ester bond between PBA and 1,2-diols, the synthesized PBA-CaCO3 exhibited good conjugation properties for CEA glycoprotein. Under optimum conditions, Ca-ISE-based aptasensing platform exhibited good electrode potential response for evaluation of target CEA, and allowed detection of CEA at a concentration as low as 7.3 pg mL(-1). Importantly, Ca-ISE-based aptasensing system is readily extended to detect other disease-related glycoproteins by controlling the corresponding aptamer.
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