期刊
ANALYTICA CHIMICA ACTA
卷 1142, 期 -, 页码 1-9出版社
ELSEVIER
DOI: 10.1016/j.aca.2020.10.049
关键词
Photoelectrochemical cytosensor; Heterojunction structure; Reusability; Cancer tumor cells; Polymerized aminophenylboronic acid
资金
- National Natural Science Foundation of China [21874013, 21775016]
- Fundamental Research Funds for the Central Universities [N170502003, N170908001, N182410008-1, N2005025, N2005027]
- China Postdoctoral Science Foundation [2019M661109]
- Talent Project of Revitalizing Liaoning [XLYC1807165]
A reusable cytosensor was developed for rapid detection of circulating tumor cells (CTCs) based on excellent photoelectrochemical (PEC) characteristic of semiconductor nanoarrays. The study demonstrated that the PEC cytosensor using polymerized APBA layer coated CdS/ZnO nanorod arrays showed good sensitivity and specificity in capturing and detecting CTCs, providing a new approach for stable monitoring.
Determination of circulating tumor cells (CTCs) is crucial for cancer diagnosis and therapy at an early stage. However, extremely low concentration of CTCs in peripheral blood makes the detection of CTCs challenging. In this study, a reusable cytosensor was developed for rapid detection of CTCs based on excellent photoelectrochemical (PEC) characteristic of semiconductor nanoarrays. Using typical breast cancer cell, MCF-7 cell, as a target model, a PEC sensing platform was constructed with polymerized aminophenylboronic acid (APBA) layer coated CdS/ZnO nanorod arrays, exhibiting outstanding performance for the capture and detection of CTCs. In this design, the polymerized APBA provides abundant binding sites for capturing terminal sialic acid (SA) molecules in CTCs. As a result, the PEC cytosensor shows good sensitivity and specificity with concentrations ranging from 50 to 1.0 x 10(6) cells/mL MCF-7 cells. Moreover, the PEC cytosensor can be rapidly and effectively recovered via a short-time bias triggered cell release and subsequent repair of APBA. This study establishes a new approach to refine a PEC cytosensor for stable monitoring and provides a robust PEC electrode with high sensitivity and low cost for clinical diagnosis related to CTCs. (C) 2020 Elsevier B.V. All rights reserved.
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