期刊
AMERICAN JOURNAL OF TRANSPLANTATION
卷 21, 期 6, 页码 2056-2066出版社
WILEY
DOI: 10.1111/ajt.16387
关键词
biomarker; cell death; apoptosis; genomics; heart biology; heart transplantation; cardiology; molecular biology; rejection; acute; translational research; science
资金
- National Institute of Health Fondo de Investigaciones Sanitarias del Instituto de Salud Carlos III [PI16/01627, PI17/01925, PI17/01232, CP18/00145]
- Consorcio Centro de Investigacion Biomedica en Red, M.P. [CB16/11/00261]
- European Regional Development Fund (FEDER)
Acute rejection after heart transplantation can lead to chronic dysfunction, with disturbances in mitochondrial function playing a contributory role. An RNA sequencing analysis identified altered mitochondrial genes in serum, particularly the MCU system, which showed strong capability for detecting rejection episodes with excellent accuracy.
Acute rejection after heart transplantation increases the risk of chronic dysfunction. Disturbances in mitochondrial function may play a contributory role, however, the relationship between histological signs of rejection in the human transplanted heart and expression levels of circulating mitochondrial genes, such as the mitochondrial Ca2+ uniporter (MCU) complex, remains unexplored. We conducted an RNA-sequencing analysis to identify altered mitochondrial genes in serum and to evaluate their diagnostic accuracy for rejection episodes. We included 40 consecutive samples from transplant recipients undergoing routine endomyocardial biopsies. In total, 112 mitochondrial genes were identified in the serum of posttransplant patients, of which 28 were differentially expressed in patients with acute rejection (p < .05). Considering the receiver operating characteristic analysis with an area under the curve (AUC) >0.900 to discriminate patients with moderate or severe degrees of rejection, we found that the MCU system showed a strong capability for detection: MCU (AUC = 0.944, p < .0001), MCU/MCUR1 ratio (AUC = 0.972, p < .0001), MCU/MCUB ratio (AUC = 0.970, p < .0001), and MCU/MICU1 ratio (AUC = 0.970, p < .0001). Mitochondrial alterations are reflected in peripheral blood and are capable of discriminating between patients with allograft rejection and those not experiencing rejection with excellent accuracy. The dysregulation of the MCU complex was found to be the most relevant finding.
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