期刊
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
卷 320, 期 3, 页码 L405-L412出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00457.2020
关键词
COVID-19; endothelial dysfunction; lung injuries; microRNA; SARS-CoV-2
资金
- National Council for Scientific and Technological Development (CNPq)
miRNAs are critical regulators of endothelial homeostasis and play a significant role in vascular diseases, including those caused by virus infections. Functional enrichment analysis identified miR-26a-5p, miR-29b-3p, and miR-34a-5p as key regulators in endothelial dysfunction and inflammatory responses in COVID-19 patients with severe lung injury and immunothrombosis.
MicroRNAs (miRNAs) are critical modulators of endothelial homeostasis, which highlights their involvement in vascular diseases, including those caused by virus infections. Our main objective was to identify miRNAs involved in the endothelial function and determine their expression in post-mortem lung biopsies of COVID-19 patients with severe respiratory injuries and thrombotic events. Based on functional enrichment analysis, miR-26a-5p, miR-29b-3p, and miR-34a-5p were identified as regulators of mRNA targets involved in endothelial and inflammatory signaling pathways, as well as viral diseases. A miRNA/mRNA network, constructed based on protein-protein interactions of the miRNA targets and the inflammatory biomarkers characterized in the patients, revealed a dose interconnection of these miRNAs in association to the endothelial activation/dysfunction. Reduced expression levels of selected miRNAs were observed in the lung biopsies of COVID-19 patients (n = 9) compared to the Controls (n = 10) (P < 0.01-0.0001). MiR-26a-5p and miR29b-3p presented the best power to discriminate these groups (area under the curve (AUC) = 0.8286, and AUC = 0.8125, respectively). The correlation analysis of the miRNAs with inflammatory biomarkers in the COVID-19 patients was significant for miR-26a-5p [IL-6 (r(2) = 0.5414), and ICAM-1 (r(2) = 0.5624)], and miR-29b-3p [IL-4 (r(2) = 0.8332) and IL-8 (r(2) = 0.2654)]. Altogether, these findings demonstrate the relevance and the non-random involvement of miR-26a-5p, miR-29b-3p, and miR-34a-5p in endothelial dysfunction and inflammatory response in patients with SARS-CoV-2 infection and the occurrence of severe lung injury and immunothrombosis.
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