Identification of a 1,274-bp promoter of a Musa acuminata L. aquaporin gene (MaPIP1;1) which confers salinity stress inducibility in transgenic Arabidopsis

Salt stress will seriously influence alt stress in banana (Musa L.) induces MaPIP1;1, which enhances the salt resistance of transgenic Arabidopsis. The promoter pMaPIP1;1, located 13,62 bp upstream of the transcriptional start site, was isolated from the banana genome, and four pMaPIP1;1::GUS fusion constructs namely M-P1, M-P2, M-P3, and M-P4 were used to transform into Arabidopsis. In this work, in order to functionally verify the promoter responds to high salt stress, we used NaCl treatment for simulating salt terms. NaCl treatment is responded to by four transformants. M-P2 (-1,274 bp to -1) showed the highest transcriptional activity among all transgenic Arabidopsis transformants. This area of the promoter endows high glucuronidase (GUS) enzyme activity according to NaCl treatment. These results will help us understand the transcriptional regulatory of MaPIP1;1, and promoter fragment M-P2 will be an ideal candidate for overexpression of salt response genes to increase plant resistance.

Automated summary

The study showed that MaPIP1;1 promoter enhances plant salt resistance, with the M-P2 fragment showing the most significant response to high salt stress among all tested constructs. This promoter fragment may be an ideal choice for enhancing plant resistance to salt stress through gene overexpression.