期刊
AGING-US
卷 13, 期 5, 页码 6565-6591出版社
IMPACT JOURNALS LLC
DOI: 10.18632/aging.202288
关键词
single-cell RNA sequencing; endometrial carcinoma; immune microenvironment; macrophage activation model; endometrial epithelial cells
资金
- National Natural Science Foundation of China [81672574, 81972438]
- Shanghai Clinical Medicine Field Project of Science and Technology Innovation Action Program [17411951600]
- Shanghai Municipal Medical and Health Discipline Construction Projects [2017ZZ02015]
This study identified multiple cell types in endometrial carcinoma, including distinct CD8(+) T cell states and different monocyte-macrophage populations, with exhausted CD8(+) T cells and macrophages enriched in tumors. Endometrial epithelial cells were classified into stem-like cells, secretory glandular cells, and ciliated cells. The study provides a detailed single-cell resolution view of endometrial carcinoma, showcasing the characteristics of endometrial epithelial cells and the cellular landscape of the tumor immune microenvironment.
Tumors are complex ecosystems harboring multiple cell types which might play a critical role in tumor progression and treatment response. The endometrial epithelial cell identities and immune microenvironment of endometrial carcinoma (ECC) are poorly characterized. In this study, a cellular map of endometrial carcinoma was generated by profiling 30,780 cells isolated from tumor and paratumor tissues from five patients using single-cell RNA sequencing. 7 cell types in lymphocytes, 7 types in myeloid cells and 3 types in endometrial epithelial cells were identified. Distinct CD8(+) T cell states and different monocyte-macrophage populations were discovered, among which exhausted CD8(+) T cells and macrophages were preferentially enriched in tumor. Both CD8(+) T cells and macrophages comport with continuous activation model. Gene expression patterns examination and gene ontology enrichment analysis of endometrial epithelial cells revealed 3 subtypes: stem like cells, secretory glandular cells and ciliated cells. Overall, our study presents a view of endometrial carcinoma at single-cell resolution that reveals the characteristics of endometrial epithelial cells in the endometrium, and provides a cellular landscape of the tumor immune microenvironment.
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