期刊
CZECH JOURNAL OF GENETICS AND PLANT BREEDING
卷 52, 期 2, 页码 77-82出版社
CZECH ACADEMY AGRICULTURAL SCIENCES
DOI: 10.17221/155/2015-CJGPB
关键词
AtCIPK23; K+ uptake; low-K+ tolerance; potassium; tobacco
资金
- China National Tobacco Corporation [110201402004]
- Shanghai Tobacco Group Co., Ltd [SZBCW2014-01065]
Tobacco (Nicotiana tabacum) has a relatively high requirement for potassium (K+). However, the molecular basis of tolerance to low-K+ stresses in tobacco still remains unknown. Here, we report the role of a member of the A. thaliana CBL (calcineurin B-like) interacting protein kinase (CIPK) family, AtCIPK23, in low-K+ stress responses in tobacco. Molecular analyses revealed that the AtCIPK23 gene was successfully transferred into a tobacco cultivar K326 via Agrobacterium tumefaciens-mediated transformation. Overexpression of AtCIPK23 in tobacco resulted in increased low-K+ tolerance, which was demonstrated by higher dry biomass, longer primary root length, higher K+ content and better growth status of transgenic tobacco plants compared to controls when both were treated in low-K+ MS medium and low-K+ hydroponics. Moreover, transgenic lines conferred tolerance to low-K+ stress by increasing the K+ uptake rate under low-K+ conditions. Taken together, these results provide evidence that AtCIPK23 may be involved in the CBL-CIPK signalling network in tobacco responses to low-K+ stress.
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